Pfu PCR Master Mix, 2x

The biotechrabbit Pfu PCR Master Mix is a perfect choice for a fast high fidelity PCR reaction set up without the need for calculation, multiple pipetting steps and buffer optimization. It is designed for routine high throughput high fidelity PCR amplifications of up to 4kb size DNA targets.

The 2x Pfu PCR Master Mix contains Pfu DNA polymerase, highest quality dNTPs and optimal PCR reaction buffer components, thus only the template and PCR primers have to be added. The final reaction volume should be reached with the pure PCR grade water.

For the most demanding applications the supplied PCR additive 5x PCR Helper can be used. It is not necessary to use for every reaction, but in many cases it helps to achieve even better results with templates having high GC content and complex structures.

The biotechrabbit Pfu DNA Polymerase is a highly purified recombinant thermostable proofreading DNA polymerase. The enzyme is isolated from E. coli carrying a plasmid with the cloned Pyrococcus furiosus DNA polymerase gene.

Pfu DNA Polymerase has about ten times higher accuracy compared to Taq DNA Polymerase.

The enzyme catalyzes template-dependent nucleotides polymerization in the 5'-3' direction, additionally it exhibits 3'-5' exonuclease (proofreading) activity, what allows the correction of nucleotide incorporation errors.

The enzyme has no 5'-3' exonuclease activity and no detectable reverse transcriptase activity.

The use of dUTP and dITP is not recommended with Pfu, because Pfu binding to DNA templates with uracil and hypoxanthine stalls DNA synthesis.

Pfu DNA Polymerase produces blunt ended PCR products suitable for blunt cloning.